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					<h2 class="elementor-heading-title elementor-size-default">Prueba de proteína C reactiva (PCR)</h2>				</div>
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									<p style="text-align: justify;">La <strong>PCR</strong>, siglas en inglés de &#8216;Reacción en Cadena de la Polimerasa&#8217;, es una prueba de diagnóstico que permite detectar un fragmento del material genético de un patógeno. En la pandemia de coronavirus, como en tantas otras crisis de salud pública relacionadas con enfermedades infecciosas, se está utilizando para determinar si una persona está infectada o no con coronavirus. A esta herramienta se están sumando en los últimos días los test de diagnóstico rápido, más sencillos y rápidos.</p><p style="text-align: justify;">El examen más usado en nuestro país es el <strong>RT-PCR (Reverse Transcriptional Polymerase chain reaction)</strong> a tiempo real, este procedimiento de detección de <span style="color: #0000ff;"><a style="color: #0000ff;" href="https://prevencionsaludproactiv.com/2020/04/25/covid-19/">Covid-19</a></span> tiene un tiempo de prueba y funciona a través de una toma de muestras de secreciones de las vías respiratorias, con la técnica de hisopado de cavidades nasofaringe y orofaringe. Muestras que pasan por un proceso en el que se extrae el material genético para recuperar el ARN viral para luego ser convertido en ADN mediante la enzima Transcriptasa inversa.</p><script async src="https://pagead2.googlesyndication.com/pagead/js/adsbygoogle.js?client=ca-pub-4254951495333536" 
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</script><p style="text-align: justify;">Continuando con el proceso se aplica un PCR (<em>polymerase chain reaction</em>) en el que otra enzima toma este ADN y replica un trozo en una reacción controlada por un computador mediante ciclos de temperatura. Esta enzima genera progresivamente un gran número de copias del ADN, y cada vez que genera una copia emite una pequeña fluorescencia que es detectada a tiempo real por el computador.</p><p style="text-align: justify;">Finalmente, si hay presencia de los genes del virus, la muestra entregará cierto grado de fluorescencia que detecta la máquina lectora. Si el virus no está presente, entonces la máquina no detectará fluorescencia.</p><p style="text-align: justify;"><img data-amp-lightbox="true" lightbox="true" class="aligncenter wp-image-13498 size-full" src="https://i0.wp.com/prevencionsaludproactiv.com/wp-content/uploads/2020/12/fotonoticia_20200502075935_1024.jpg?resize=640%2C427&#038;ssl=1" alt="" width="640" height="427" /></a></p><p style="text-align: justify;">En Chile, es el <strong>Instituto de Salud Pública (ISP)</strong>, el único laboratorio de referencia nacional para la confirmación del nuevo coronavirus. Por lo tanto, si se detecta un caso sospechoso (<strong>persona con síntomas respiratorios</strong> y <strong>proveniente de un país con alto número de contagios</strong>), el individuo debe ser atendido por un centro de salud (público o privado) donde primero se descarta la presencia de otros patógenos respiratorios como, neumococo, virus sincicial, influenza u otros coronavirus que producen resfrío.</p><p style="text-align: justify;">Si los test más habituales para detectar <strong>enfermedades respiratorias son negativos,</strong> entonces se extrae una muestra que debe ser enviada al ISP para un análisis más específico.</p><p style="text-align: justify;"> Las muestras que se estudian son de <strong>secreciones de las vías respiratorias</strong> (altas o bajas) lo que incluye el aspirado nasofaríngeo, secreciones bronquiales o pulmonares (como expectoración, aspirado endotraqueal o lavado bronquioalveolar). Esta última es una muestra que se indica cuando existen casos de neumonía grave.</p><p style="text-align: justify;">La técnica que se utiliza es de <strong>biología molecular</strong> en la que se detectan los genes del virus (<strong>detección genómica</strong>). Esta información que es exclusiva y específica para este nuevo coronavirus por lo que <strong>no se puede confundir con otros coronavirus y otros virus respiratorios.</strong></p><p style="text-align: justify;"><strong>Dos exámenes</strong></p><p style="text-align: justify;">Par la confirmación diagnóstica el ISP cuenta con <strong>dos tipos de exámenes</strong>. Uno es el <strong>secuenciamiento genómico</strong> que busca detecta una región específica del genoma del virus en la muestra y, desde ayer, un segundo examen denominado <strong>PCR (reacción de polimerasa en cadena)</strong> que es más rápido que el primero y que amplifica el material genético del virus y lo busca en la muestra.</p><p style="text-align: justify;"><strong>El examen por PCR es una técnica creada y elaborada en el ISP</strong> gracias a que las autoridades chinas hicieron pública la secuenciación genómica del virus.</p><p style="text-align: justify;">Actualmente en Chile se están haciendo los dos exámenes para confirmar los resultados: se practica en PCR que está listo en 24 horas y también el secuenciamiento genético que confirma ese resultado pero entre 48 a 72 horas.</p><p style="text-align: justify;">La <strong>PCR es una técnica molecular</strong> que permite la detección de un material genético específico. En este caso, la PCR en tiempo real implementada en el Instituto de Salud Pública, permite detectar la presencia o ausencia del material genético del virus en las muestras de los pacientes.</p><p style="text-align: justify;">La <strong>secuenciación</strong> es una técnica complementaria que, además de detectar la presencia del material genético del virus, permite analizar una región del genoma del coronavirus y confirmar que el virus no ha sufrido mutaciones o cambios genéticos. La secuenciación de ADN se realiza con tecnología de nanoporos, que alcanza una exactitud de 99,99%.</p>								</div>
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									<div><h3>Detección de antígenos o de anticuerpos</h3></div><div> </div><div>A diferencia de la PCR, estos test rápidos no identifican el ARN del virus, sino que detectan, o bien anticuerpos producidos frente al virus utilizando una muestra de sangre, que es otra manera de conocer si el paciente está o ha estado infectado, o bien proteínas del virus presentes en las muestras respiratorias de exudado nasofaríngeo. Además de la rapidez, presentan otra ventaja muy importante en el momento actual ya que pueden realizarse en el domicilio de un caso sospechoso, siempre supervisado por un profesional sanitario. </div><div> </div><div>Estos tests de diagnóstico rápido se basan en una inmunocromatografía en papel, es decir, una plataforma que tiene &#8216;pegados&#8217; las proteínas del virus para detectar anticuerpos específicos para detectar las proteínas del virus. Su funcionamiento es similar al de los test de embarazo. </div><div> </div><div>Gracias a estos test se podrá mejorar el cribado en la población y limitar los ensayos de PCR sólo a aquellos pacientes que, con sintomatología, den un resultado negativo mediantes los tests rápidos, lo que permitirá liberar profesionales y recursos en el Sistema Nacional de Salud.</div>								</div>
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							<h4>Prevención en Salud Proactiva</h4> 
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							<p>Paula Rojas Hormazábal</p> 
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							<p>Nuestra misión es proteger la vida y la salud de las personas, con enfoque en el entorno laboral. A través de programas especializados en primeros auxilios y maniobras críticas (RCP, DEA, SVB y Heimlich), educamos y capacitamos a personas y equipos para responder con seguridad ante emergencias. Además, garantizamos la continuidad y el bienestar organizacional mediante una gestión de riesgos alineada con la Ley 16.744 y los más altos estándares ISO.</p><div class="fa2752738c61f2593e5755f4d15b4d9c" data-index="2" style="float: none; margin:0px 0 0px 0; text-align:center;">
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2025/12/02/cadena-de-supervivencia-unificada-aha-2025/" class="_blank" target="_blank" >Cadena de Supervivencia Unificada AHA 2025</a></h4>
<div class="pt-cv-content">La Nueva Cadena de Supervivencia Unificada Esta cadena unificada refleja ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2025/12/02/cadena-de-supervivencia-unificada-aha-2025/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2025/10/29/guias-aha-2025-para-rcp-y-ace-actualizaciones/" class="_blank" target="_blank" >Guías AHA 2025 para RCP y ACE Actualizaciones</a></h4>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2025/10/25/phtls-10-a-edicion-cambios-en-la-atencion-del-trauma/" class="_blank" target="_blank" >PHTLS 10.ª Edición: Cambios en la Atención del Trauma</a></h4>
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<div class="pt-cv-content">El lavado de manos clínico, también conocido como lavado de ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2024/08/08/lavado-de-manos-clinico-en-salud/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2022/08/06/carro-de-paro-o-reanimacion/" class="_blank" target="_blank" >Carro de Paro o Reanimación</a></h4>
<div class="pt-cv-content">El carro rojo, de paro o de reanimación es una unidad ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2022/08/06/carro-de-paro-o-reanimacion/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2022/05/12/rcp-en-tiempos-de-covid-19/" class="_blank" target="_blank" >RCP en Tiempos de COVID-19</a></h4>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2022/02/25/tendinitis-2/" class="_blank" target="_blank" >Tendinitis</a></h4>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2022/02/24/superficies-de-trabajo/" class="_blank" target="_blank" >Superficies de Trabajo</a></h4>
<div class="pt-cv-content">CHARLA DE 5 MINUTOS Personas que han visualizado la charla ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2022/02/24/superficies-de-trabajo/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2022/02/24/riesgo-electrico/" class="_blank" target="_blank" >Riesgo Eléctrico</a></h4>
<div class="pt-cv-content">Los riesgos eléctricos son situaciones en las que existe la ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2022/02/24/riesgo-electrico/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2022/02/24/primeros-auxilios-2/" class="_blank" target="_blank" >Primeros Auxilios</a></h4>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2022/02/20/rcp-solo-con-las-manos/" class="_blank" target="_blank" >RCP Solo con las Manos</a></h4>
<div class="pt-cv-content">CHARLA DE 5 MINUTOS La reanimación cardiopulmonar (RCP) es una ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2022/02/20/rcp-solo-con-las-manos/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2022/01/18/maniobra-de-heimlich-charla-de-seguridad/" class="_blank" target="_blank" >Maniobra de Heimlich</a></h4>
<div class="pt-cv-content">Maniobra de Heimlich en casos de atragantamiento La Maniobra de ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2022/01/18/maniobra-de-heimlich-charla-de-seguridad/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2021/12/22/aspectos-destacados-de-actualizacion-en-primeros-auxilios-aha/" class="_blank" target="_blank" >Aspectos Destacados de Actualización en Primeros Auxilios AHA</a></h4>
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<div class="pt-cv-content">El aumento del estrés, el estilo de vida sedentario, el ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2021/12/07/importancia-de-tener-un-dea-en-el-lugar-de-trabajo/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2021/12/07/ley-numero-21-156-desfibriladores-dea/" class="_blank" target="_blank" >Ley N° 21.156 Desfibriladores (DEA)</a></h4>
<div class="pt-cv-content">D.S 56 APRUEBA REGLAMENTO SOBRE LA OBLIGACIÓN DE DISPONER DE ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2021/12/07/ley-numero-21-156-desfibriladores-dea/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2021/11/19/escala-de-eva-en-enfermeria/" class="_blank" target="_blank" >Escala de EVA en Enfermería</a></h4>
<div class="pt-cv-content">La escala del dolor EVA en enfermería (Escala Visual Analógica), ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2021/11/19/escala-de-eva-en-enfermeria/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2021/11/18/estados-de-inconsciencia/" class="_blank" target="_blank" >Estados de Inconsciencia</a></h4>
<div class="pt-cv-content">Estado de inconsciencia El estado o situación en que una ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2021/11/18/estados-de-inconsciencia/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2021/11/03/conducta-pas/" class="_blank" target="_blank" >Conducta PAS</a></h4>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2021/02/01/fractura/" class="_blank" target="_blank" >Fracturas</a></h4>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2020/11/21/asma/" class="_blank" target="_blank" >Asma</a></h4>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2020/11/21/quemaduras/" class="_blank" target="_blank" >Quemaduras</a></h4>
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<div class="pt-cv-content">RCP extrahospitalario en contexto COVID-19 La RCP en tiempos de ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2020/11/19/reanimacion-cardiopulmonar-en-covid-19/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2020/11/13/maniobra-de-heimlich-en-ninos/" class="_blank" target="_blank" >Maniobra de Heimlich en Niños, Lactantes y Embarazadas</a></h4>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2020/11/11/primeros-auxilios/" class="_blank" target="_blank" >Charla de Primeros Auxilios</a></h4>
<div class="pt-cv-content">Charla de 5 minutos en Primeros Auxilios El objetivo de ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2020/11/11/primeros-auxilios/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2021/11/27/fleboclisis/" class="_blank" target="_blank" >Fleboclisis</a></h4>
<div class="pt-cv-content">Qué es fleboclisis: La fleboclisis o venoclisis consiste en la canulación de una vena de manera tal que, a través ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2021/11/27/fleboclisis/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2021/09/22/caso-clinico-infarto-agudo-de-miocardio-iam/" class="_blank" target="_blank" >Caso Clínico Infarto Agudo de Miocardio IAM</a></h4>
<div class="pt-cv-content">Antecedentes Paciente de 57 años, con antecedentes de tabaquismo crónico, ingresa al servicio de urgencia con dolor retroesternal opresivo intenso ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2021/09/22/caso-clinico-infarto-agudo-de-miocardio-iam/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2021/04/17/paciente-con-acv-isquemico/" class="_blank" target="_blank" >Paciente con ACV Isquémico</a></h4>
<div class="pt-cv-content">ACV ISQUÉMICO El accidente cerebrovascular ACV isquémico generalmente se produce por la obstrucción de una arteria que va al cerebro; ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2021/04/17/paciente-con-acv-isquemico/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2021/04/07/caso-clinico-pae/" class="_blank" target="_blank" >Caso Clínico PAE</a></h4>
<div class="pt-cv-content">CASO CLÍNICO El siguiente PAE y caso clínico, lo realicé con trabajo y dedicación, aplicando los conocimientos adquiridos durante mi ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2021/04/07/caso-clinico-pae/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2021/04/03/importancia-de-los-electrolitos/" class="_blank" target="_blank" >Importancia de los Electrolitos</a></h4>
<div class="pt-cv-content">Los electrolitos Los electrolitos son minerales naturales que contienen una carga eléctrica. Los electrolitos se encuentran en tu sangre, tu ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2021/04/03/importancia-de-los-electrolitos/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2021/03/27/sueros-en-enfermeria/" class="_blank" target="_blank" >Soluciones Parenterales en Enfermería</a></h4>
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<h4 class="pt-cv-title"><a href="https://prevencionsaludproactiv.com/2021/02/23/proceso-de-atencion-de-enfermeria-pae/" class="_blank" target="_blank" >Proceso de Atención de Enfermería PAE</a></h4>
<div class="pt-cv-content">Concepto PAE El <;b data-path-to-node="0" data-index-in-node="3">;Proceso de Atención de Enfermería (PAE)<;/b>; es el método sistemático, organizado y científico que utilizamos ...<br /><div class="pt-cv-rmwrap"><a href="https://prevencionsaludproactiv.com/2021/02/23/proceso-de-atencion-de-enfermeria-pae/" class="_blank pt-cv-readmore btn btn-success" target="_blank">Leer Más</a></div></div></div></div></div></div></div></div></div></div><div class="widget_text awac-wrapper"><div class="widget_text awac widget custom_html-110"><div class="textwidget custom-html-widget"><script async src="https://pagead2.googlesyndication.com/pagead/js/adsbygoogle.js?client=ca-pub-4254951495333536" 
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